The coronavirus disease-19 (COVID-19) pandemic emerged as a global health threat. Various studies

conducted in different healthcare systems/setups indicated that healthcare workers are at increased risk for COVID-19. To

find out the seroprevalence of COVID-19 in staff members of different institutions of Khyber Medical University,

Peshawar.

This descriptive cross-sectional study was conducted at Khyber medical university Peshawar. The sampling

was done from February 2021 to March 2021. A total of 352 samples were collected. Data was collected on a preformed

questionnaire after obtaining written informed consent. 3ml of blood was collected in gel top to determine IgM and IgG

antibody levels by indirect manual ELISA technique. Data were recorded and analyzed in SPSS version 24.

Amongst the staff of KMU, a total of 54.8% cases were reported as asymptomatic, 14.4% as confirmed (with the

highest IgG seropositivity of 86.2%), 14.4% as suspected, and 16.1% as probable cases of COVID-19. While out of

confirmed cases of COVID-19, 86.2% with mild, 7.8% with moderate, and 5.8% cases with severe disease were

documented, respectively. 1.7% of samples were seropositive for IgM antibodies, while 58.2% were found positive for

IgG antibodies. Amongst the KMU's Faculty, the Administrative Staff group was found to have the highest seropositivity

of IgG antibodies of about 60.7%.

KMU staff was at significant risk of getting in contact with the virus due to the operationalization of the

university throughout the pandemic as well as the deployment of university staff at the public health reference laboratory

(PHRL). Follow-up studies are required to assess the longevity of both IgM & IgG antibodies and to explore further the

kinetics of antibodies