Original Research

Diagnostic Markers in Specific Subsets of Infertile Males: Exploring the Significance of Fructose, Seminal histone Deacetylase, and Serum Reproductive Hormones

DOI https://doi.org/10.70302/jpsim.v5i2.2434
Received: 29 Jan 2024 Accepted: 06 Mar 2024 Published Online: 07 Jun 2024

Abstract

Objective

The objective of the study was to evaluate the diagnostic markers in specific subsets of infertile males and
exploring the significance of fructose, seminal histone deacetylase, and serum reproductive hormones.

Methods

This investigation encompassed 80 infertile males and an equal number of fertile males (used as controls).
Anthropometric measurements such as weight and height were taken to compute the weight-to-height ratio (BMI). Blood
from all participants was collected through venipuncture, undergone coagulation, followed by centrifugation to isolate
serum for analysis. This analysis focused on the concentrations of follicle stimulating hormone (FSH), testosterone, and
luteinizing hormone (LH). Additionally, spermatozoa samples were collected via sexually self-stimulation to assess
spermatozoa quality, activity of histone deacetylase and levels of seminal fructose.

Results

Comparisons between different subgroups of infertile men yielded the following outcomes: body mass index,
height and weight were similar across the groups; semen volume and the hormones FSH and LH were significantly higher
than expected, higher levels of sugar (fructose) and specific enzyme activity (HDAC) in semen, but lower sperm count,
movement (motility), and male hormone (testosterone) levels. Furthermore, a strong and positive correlation was
identified between sugar (fructose) and specific enzyme activity (HDAC). The study did not find a strong or meaningful
relationship between HDAC activity and sperm motion, unlike the significant negative associations observed with sperm
count and testosterone.

Conclusion

This study suggests a promising new way to diagnose male infertility. By checking levels of sugar (fructose)
and specific enzyme activity (HDAC) in semen, alongside the usual tests, doctors could catch problems they might miss
otherwise. This could help men get the treatment they need sooner and improve their chances of having a family.

Keywords
Infertility Biomarkers SemenAnalysis SpermatozoaAbnormalities ReproductiveTractInfectionsdiagnosis

How to Cite This Article

Shah FU, Anjum J, Shoaib M, Khan A, Bashir CA, Ahmad W. Diagnostic Markers in Specific Subsets of Infertile Males: Exploring the Significance of Fructose, Seminal histone Deacetylase, and Serum Reproductive Hormones. J Pak Soc Intern Med. 2024;5(2):521–527. doi:10.70302/jpsim.v5i2.2434

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Conflict of Interest

All authors declare no competing interests.

Disclosed in accordance with ICMJE and COPE guidelines.

Funding

No specific funding was received for this research.

Funder information follows the Crossref Funder Registry standard.

References

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CC BY CC BY 4.0 This article is published under the Creative Commons Attribution 4.0 International License. May be shared and adapted with attribution. © 2024 Journal of Pakistan Society of Internal Medicine.