Diagnostic Markers in Specific Subsets of Infertile Males: Exploring the Significance of Fructose, Seminal histone Deacetylase, and Serum Reproductive Hormones


  • Author- Farid Ullah Shah, Jahangir Anjum, Muhammad Shoaib, Afsar Khan, Chaudhary Ammar Bashir, Wasim Ahmad


Objective: The objective of the study was to evaluate the diagnostic markers in specific subsets of infertile males and exploring the significance of fructose, seminal histone deacetylase, and serum reproductive hormones.


This investigation encompassed 80 infertile males and an equal number of fertile males (used as controls). Anthropometric measurements such as weight and height were taken to compute the weight-to-height ratio (BMI). Blood from all participants was collected through venipuncture, undergone coagulation, followed by centrifugation to isolate serum for analysis. This analysis focused on the concentrations of follicle stimulating hormone (FSH), testosterone, and luteinizing hormone (LH). Additionally, spermatozoa samples were collected via sexually self-stimulation to assess spermatozoa quality, activity of histone deacetylase and levels of seminal fructose.


 Comparisons between different subgroups of infertile men yielded the following outcomes: body mass index, height and weight were similar across the groups; semen volume and the hormones FSH and LH were significantly higher than expected, higher levels of sugar (fructose) and specific enzyme activity (HDAC) in semen, but lower sperm count, movement (motility), and male hormone (testosterone) levels. Furthermore, a strong and positive correlation was identified between sugar (fructose) and specific enzyme activity (HDAC). The study did not find a strong or meaningful relationship between HDAC activity and sperm motion, unlike the significant negative associations observed with sperm count and testosterone.


 This study suggests a promising new way to diagnose male infertility. By checking levels of sugar (fructose) and specific enzyme activity (HDAC) in semen, alongside the usual tests, doctors could catch problems they might miss otherwise. This could help men get the treatment they need sooner and improve their chances of having a family