Original Research

Detection of Extended Spectrum Beta Lactamase Producing Enterobacteriaceae and Their Antibiotic Susceptibility Testing

Javairia Saeed1
Nishtar Medical University Multan
Asghar Javaid1
Nishtar Medical University Multan
Rashda Shabbir2
CMH Multan Institue of Medical Sciences Multan
Romah Ashfaq3
Multan Medical and Dental College Multan
Blossom Neelam1
Nishtar Medical University Multan
Sumera Malik2
CMH Multan Institue of Medical Sciences Multan

✉ Correspondence: Javairia Saeed Email: javariasaeed98@yahoo.com

DOI https://doi.org/10.70302/jpsim.v5i1.2415
Volume: 5, Issue 1 (Women Issue) Pages: 427–432 Published: 28 Feb 2024
Received: 06 Oct 2023 Accepted: 14 Dec 2023 Published Online: 28 Feb 2024

Abstract

Objective

To determine the antibiotic susceptibility pattern of Enterobacteriaceae and incidence of extended-spectrum beta-lactamases-producing Enterobacteriaceae.

Methods

This descriptive study consisted of a sample size of 200 Enterobacteriaceae isolates, was conducted in the Microbiology section of the Pathology department of Nishtar Medical University Multan from November 2020 to November 2021 . Blood, urine, and pus specimens were collected from patients of all age groups and either sex, after taking informed written consent. Identification was carried out by conventional biochemical tests and confirmed by Analytical Profile Index 20 E. Kirby-Bauer disc diffusion method was used to evaluate the sensitivity of bacterial isolates against different antibiotics. Identification of Extended Spectrum Beta Lactamase (ESBL) production was carried out by double-disc diffusion phenotypic confirmatory method.

Results

Among 200 culture-positive samples for Enterobacteriaceae, 77(38.5%) were from pus samples, 51(25.5%) were from blood and 72(36.0%) were from urine samples. The highest frequency of 111(55.5%) was identified as Eschercia coli. ESBL positive strains of enteric bacteria revealed in 29.5% of organisms. Patterns of drug susceptibility in ESBL negative showed highest resistance against Cefuroxime and Cefotaximeas 125/141 and 118/141 respectively while 59/59 for each in ESBL positive group.

Conclusion

A high proportion of ESBL- producing isolates belonging to Enterobacteriaceae were obtained from pus samples considering wound infections followed by urine and blood. The frequency of Eschercia coli remained the highest among ESBL and non-ESBL producer Enterobacteriaceae followed by Proteus and Enterobacter in this study. Meropenem and amikacin remained highly effective drugs for both groups followed by chloramphenicol and piperacillin/tazobactam.

Keywords
Resistance Antibiotic ESBL β-lactamases E. coli Enterobacteriaceae.

How to Cite This Article

Saeed J, Javaid A, Shabbir R, Ashfaq R, Neelam B, Malik S. Detection of Extended Spectrum Beta Lactamase Producing Enterobacteriaceae and Their Antibiotic Susceptibility Testing. J Pak Soc Intern Med. 2024;5(1):427–432. doi:10.70302/jpsim.v5i1.2415

Conflict of Interest

All authors declare no competing interests.

Disclosed in accordance with ICMJE and COPE guidelines.

Funding

No specific funding was received for this research.

Funder information follows the Crossref Funder Registry standard.

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CC BY CC BY 4.0 This article is published under the Creative Commons Attribution 4.0 International License. May be shared and adapted with attribution. © 2024 Journal of Pakistan Society of Internal Medicine.
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